Evaluation of infections in orthopedic patients using next-generation sequencing

Introduction Culture tests are used to diagnose infections, but there various problems such as low sensitivity in detecting infections orthopedic cases. To address this problem, next generation sequencing (NGS) analysis, which can comprehensively search for bacterial genes, is being applied clinically. In study, we examined whether NGS analysis was useful evaluating Methods The participants were 23 patients suspected of having an infection between 2016 and 2017. Samples collected from tissues infected subjected culture the positive rates compared. We also attempted determine cutoff value analysis. Results A total 20 cases ultimately diagnosed 3 non-infections. 70%, 55%. When performed with diversity index set cut-off value, 75% Simpson index. 90% when using index, a combination OTUs (operational taxonomic units) value. Conclusion showed excellent specificity compared tests. therefore modality assessing